25/07/29Emkhakheni we-bioluminescence imaging ye-high-throughput kanye nokutholwa kokukhanya okuphansi kwemboni ngesivinini esiphezulu, ukuzuza ibhalansi efanele phakathi kwejubane lokucabanga nokuzwela sekuyisikhathi eside kuyibhodlela eliwumgogodla elikhawulela inqubekelaphambili yezobuchwepheshe. Izixazululo zezithombe zomugqa womugqa noma wendawo ngokuvamile zibhekana nokuhwebelana okunzima, okwenza kube inselele ukugcina kokubili ukusebenza kahle kokutholwa nokusebenza kwesistimu. Ngenxa yalokhu, ukuthuthukiswa kwezimboni kube nzima kakhulu.
Ukwethulwa kobuchwepheshe be-back-illuminated TDI-sCMOS sekuqala ukubhekana nale mikhawulo. Lobu buchwepheshe obusha abugcini nje ngokubhekana nemikhawulo ebonakalayo yokuthatha izithombe ngesivinini esiphezulu ezimweni zokukhanya okuphansi kodwa futhi bunweba ukusetshenziswa kwakho ngale kwesayensi yempilo emikhakheni yezimboni ethuthukisiwe njengokuhlolwa kwe-semiconductor kanye nokukhiqiza ukunemba. Ngalezi ntuthuko, i-TDI-sCMOS iya ngokuya isetshenziswa ezinhlelweni zokusebenza zesimanje zezithombe zemboni.
Lesi sihloko sichaza izimiso ezibalulekile ngemuva kwe-TDI imaging, ilandelela ukuvela kwayo, futhi idingida indima yayo ekhulayo ezinhlelweni zezimboni.
Ukuqonda Izimiso ze-TDI: Ukuphumelela Emfanekisweni Onamandla
I-Time Delay Integration (TDI) ubuchwepheshe bokutholwa kwesithombe obususelwe kumgomo wokuskena umugqa onikeza izici ezimbili ezibalulekile zobuchwepheshe:
Ukutholwa Okunamandla Okuvumelanayo
Ngokungafani namakhamera endawo asebenza kumjikelezo othi "stop-shot-move", izinzwa ze-TDI ziyaqhubeka ziveza izithombe ngenkathi zinyakaza. Njengoba isampuli ihamba endaweni yokubuka, inzwa ye-TDI ivumelanisa ukunyakaza kwamakholomu ephikseli nesivinini sento. Lokhu kuvumelanisa kuvumela ukuchayeka okuqhubekayo kanye nokunqwabelana kweshajethi eguqukayo yento efanayo ngokuhamba kwesikhathi, okuvumela ukuthwebula izithombe okuphumelelayo ngisho nangesivinini esikhulu.
I-TDI Imaging Demonstration: Ukunyakaza Kwesampula Okudidiyelwe kanye Nokuhlanganiswa Kwezindleko
I-Charge Domain Accumulation
Ikholomu yephikseli ngayinye iguqula ukukhanya okungenayo kube ukushaja kagesi, okube sekucutshungulwa ngezigaba eziningi zokufunda amasampula. Le nqubo yokuqoqa eqhubekayo ithuthukisa ngempumelelo isignali ebuthakathaka nge-factor engu-N, lapho i-N imelela inani lamazinga okuhlanganiswa, ukuthuthukisa isilinganiso sesignali-kuya-noise (SNR) ngaphansi kwezimo zokukhanya ezilinganiselwe.
Umfanekiso Wekhwalithi Yesithombe Ezigabeni Ezihlukene ze-TDI
Ukuvela kobuchwepheshe be-TDI: Ukusuka ku-CCD kuya ku-Back-Illuminated sCMOS
Izinzwa ze-TDI zaqale zakhiwa ku-CCD noma ezisekelweni ze-CMOS ezikhanyiswe ngaphambili, kodwa zombili lezi zakhiwo zinemikhawulo lapho zisetshenziswa ekucabangeni okusheshayo nokuphansi kokukhanya.
TDI-CCD
Izinzwa ze-TDI-CCD ezikhanyiselwe emuva zingafinyelela ukusebenza kahle kwe-quantum (QE) eduze kwama-90%. Kodwa-ke, i-serial readout rchitecture yabo ikhawulela isivinini sokucabanga—izilinganiso zomugqa ngokuvamile zihlala zingaphansi kuka-100 kHz, nezinzwa zokuxazulula okungu-2K ezisebenza cishe ku-50 kHz.
I-Front-Illuminated TDI-CMOS
Izinzwa ze-TDI-CMOS ezikhanyisiwe ngaphambili zinikeza isivinini sokufunda esisheshayo, ngamanani womugqa wokucaca ongu-8K afinyelela ku-400 kHz. Kodwa-ke, izici zesakhiwo zikhawulela i-QE yazo, ikakhulukazi kububanzi obufushane be-wavelength, ngokuvamile obugcina bungaphansi kwama-60%.
Intuthuko ephawulekayo yeza ngo-2020 ngokukhululwa kwe-Tucsen'sIkhamera ye-Dhyana 9KTDI sCMOS, ikhamera ye-TDI-sCMOS ekhanyiswe ngemuva. Kuphawula ukweqa okubalulekile ekuhlanganiseni ukuzwela okuphezulu nokusebenza kwe-TDI enesivinini esikhulu:
-
Ukusebenza kahle kwe-Quantum: i-QE ephakeme engu-82%—iphakeme cishe ngo-40% kunezinzwa ezivamile ze-TDI-CMOS ezikhanyiswe ngaphambili, okuyenza ilungele ukuthwebula izithombe ezinokukhanya okuphansi.
-
Isilinganiso Somugqa: 510 kHz ekulungisweni kwe-9K, okuhumushela ekuphumeni kwedatha okungu-4.59 gigaphixels ngomzuzwana.
Lobu buchwepheshe buqale ukusetshenziswa ekuskeneni kwe-high-throughput fluorescence, lapho ikhamera ithwebule khona isithombe esingu-2-gigapixel sesampula ye-fluorescent engu-30 mm × 17 mm kumasekhondi angu-10.1 ngaphansi kwezimo zesistimu ethuthukisiwe, okubonisa izinzuzo ezinkulu ngesivinini sokucabanga kanye nokwethembeka kwemininingwane kumasistimu avamile wokuskena endaweni.
Isithombe: I-Dhyana 9KTDI enesiteji sezimoto i-Zaber MVR
Inhloso: 10X Isikhathi sokutholwa: 10.1s Isikhathi sokuchayeka: 3.6ms
Usayizi wesithombe: 30mm x 17mm 58,000 x 34,160 amaphikseli
Izinzuzo ezibalulekile ze-TDI Technology
Ukuzwela Okuphezulu
Izinzwa ze-TDI ziqongelela amasignali phezu kokuchayeka okuningi, okuthuthukisa ukusebenza kokukhanya okuphansi. Ngezinzwa ze-TDI-sCMOS ezikhanyiselwe emuva, ukusebenza kahle kwe-quantum ngaphezu kuka-80% kuyafezeka, okusekela imisebenzi enzima efana ne-fluorescence imaging kanye nokuhlolwa kwenkundla emnyama.
Ukusebenza Kwejubane Eliphezulu
Izinzwa ze-TDI ziklanyelwe ukuthwebula izithombe eziphezulu, zithwebula izinto ezihamba ngokushesha ngokucaca okuhle kakhulu. Ngokuvumelanisa ukufundwa kwe-pixel nokunyakaza kwento, i-TDI icishe isuse ukufiphala kokunyakaza futhi isekele ukuhlola okusekelwe kumthumeli, ukuskena kwesikhathi sangempela, nezinye izimo zomphumela ophezulu.
I-Signal-to-Noise Ratio (SNR) ethuthukisiwe
Ngokuhlanganisa amasiginali kuzo zonke izigaba eziningi, izinzwa ze-TDI zingakhiqiza izithombe zekhwalithi ephezulu ezinokukhanya okuncane, zinciphise ubungozi bokwenza izithombe kumasampula ezinto eziphilayo futhi zinciphise ukucindezeleka okushisayo ezintweni ezibucayi.
Ukunciphisa Ukuthinteka Kokuphazamiseka Kwe-Ambient
Ngokungafani nezinhlelo zokuskena kwendawo, izinzwa ze-TDI azithintwa kancane ukukhanya kwe-ambient noma izibonisi ngenxa yokuchayeka kwazo okuvumelanisiwe komugqa nomugqa, okuzenza ziqine kakhulu ezindaweni eziyinkimbinkimbi zezimboni.
Isibonelo sohlelo lokusebenza: Ukuhlolwa kwe-wafer
Emkhakheni we-semiconductor, amakhamera e-scan sCMOS ayevame ukusetshenziselwa ukutholwa kokukhanya okuphansi ngenxa yejubane nokuzwela kwawo. Nokho, lawa masistimu angaba nezihibe:
-
Inkambu Yokubuka Elinganiselwe: Ozimele abaningi badinga ukuthungwa ndawonye, okuholela ezinqubweni ezidla isikhathi.
-
Ukuskena Okunensayo: Ukuskena ngakunye kudinga ukulinda ukuthi isiteji sizinze ngaphambi kokuthwebula isithombe esilandelayo.
-
Ukuthunga Ama-Artifact: Izikhala zesithombe nokungahambisani kuthinta ikhwalithi yokuskena.
I-TDI imaging isiza ukubhekana nalezi zinselele:
-
Ukuskena Okuqhubekayo: I-TDI isekela ukuskena okukhulu, okungaphazamiseki ngaphandle kwesidingo sokuthungwa kozimele.
-
Ukutholwa Okushesha Kakhulu: Amazinga aphezulu omugqa (kufika ku-1 MHz) asusa ukubambezeleka phakathi kokuthwebula.
-
Ukufana Kwesithombe Okuthuthukisiwe: Indlela ye-TDI yokuskena umugqa inciphisa ukuhlanekezelwa kokubuka futhi iqinisekisa ukunemba kwejometri kuso sonke iskena.
I-TDI VS Area Scan
Umfanekiso: I-TDI inika amandla inqubo yokutholwa eqhubekayo futhi ebushelelezi
Ikhamera ye-Tucsen's Gemini 8KTDI sCMOS isebenze kahle ekuhloleni i-wafer ye-ultraviolet ejulile. Ngokokuhlola kwangaphakathi kwe-Tucsen, ikhamera ifinyelela ku-63.9% QE ku-266 nm futhi igcina izinga lokushisa le-chip lizinzile ku-0°C ngaphezu kokusetshenziswa okunwetshiwe—okubalulekile ezinhlelweni ezizwela i-UV.
Ukwandisa Ukusetshenziswa: Ukusuka Ekufanekiseni Okukhethekile kuya Ekuhlanganisweni Kwesistimu
I-TDI ayisakhawulelwe kuzinhlelo zokusebenza ze-niche noma ukuhlolwa kwebhentshimakhi. Ukugxila kushintshele ekuhlanganisweni okungokoqobo nezinhlelo zezimboni.
Uchungechunge lwe-Tucsen's Gemini TDI lunikeza izinhlobo ezimbili zezixazululo:
1. Amamodeli we-Flagship: Idizayinelwe amakesi okusetshenziswa okuthuthukile njengokuhlola iwafa engaphambili kanye nokutholwa kokonakala kwe-UV. Lawa mamodeli abeka phambili ukuzwela okuphezulu, ukuzinza, kanye nokusebenza.
2. Izinhlobonhlobo Ezihlangene: Amandla amancane, apholile emoyeni, futhi aphansi—afaneleka kakhulu kumasistimu ashumekiwe. Lawa mamodeli ahlanganisa i-CXP (CoaXPress) ukuxhumana kwesivinini esikhulu sokuhlanganiswa okuhlelekile.
Kusukela ekucabangeni komphumela ophezulu kusayensi yempilo kuya ekuhlolweni okunembayo kwe-semiconductor, i-TDI-sCMOS ekhanyisiwe ngemuva idlala indima ebaluleke kakhulu ekuthuthukiseni ukugeleza komsebenzi wesithombe.
Imibuzo Evame Ukubuzwa
Q1: Isebenza kanjani i-TDI?
I-TDI ivumelanisa ukudluliswa kweshaja kuyo yonke imigqa yamaphikseli nokunyakaza kwento. Njengoba into ihamba, umugqa ngamunye uqongelela okunye ukuchayeka, okwandisa ukuzwela, ikakhulukazi ekusetshenzisweni kokukhanya okuphansi kanye nesivinini esikhulu.
Q2: Bungasetshenziswa kuphi ubuchwepheshe be-TDI?
I-TDI ilungele ukuhlolwa kwe-semiconductor, ukuskena kwe-fluorescence, ukuhlolwa kwe-PCB, nezinye izinhlelo zokusebenza zokucabanga ezinokulungiswa okuphezulu, ezinesivinini esikhulu lapho ukufiphala kokunyakaza nokukhanya okuphansi kuwukukhathazeka.
Q3: Yini okufanele ngiyicabangele lapho ngikhetha ikhamera ye-TDI yezicelo zezimboni?
Lapho ukhetha ikhamera ye-TDI, izici ezibalulekile zihlanganisa isilinganiso somugqa, ukusebenza kahle kwe-quantum, ukulungiswa, impendulo ye-spectral (ikakhulukazi yezinhlelo zokusebenza ze-UV noma ze-NIR), nokuzinza kwe-thermal.
Ukuze uthole incazelo enemininingwane yokuthi ubalwa kanjani isilinganiso somugqa, bheka ku-athikili yethu:
Uchungechunge lwe-TDI – Ungabala Kanjani Imvamisa Yomugqa Wekhamera
I-Tucsen Photonics Co., Ltd. Wonke amalungelo agodliwe. Uma ucaphuna, sicela wazise umthombo:www.tucsen.com
