22/03/04Abtract
Yawancin binciken bincike na yau da kullun sun yi nazarin koma bayan da aka haifar da tabar heroin da hodar iblis da ake nema a cikin nau'ikan dabbobi, amma yawancin waɗannan karatun sun yi nazarin magani ɗaya ne kawai a lokaci guda. A cikin masu shan ɗan adam, duk da haka, yawan amfani da hodar iblis da tabar heroin ya zama ruwan dare. Mun yi amfani da tsarin koma baya na sarrafa kansa a cikin berayen don tantance kamanceceniya da bambance-bambance a wuraren kwakwalwa da aka kunna yayin da aka haifar da sake dawo da tabar heroin da neman hodar iblis. Gabaɗaya, sakamakon ya nuna cewa PL na iya zama yanki na kwakwalwa na gama gari da ke cikin duka tabar heroin da hodar iblis yayin amfani da polydrug; ba a sami ingantaccen kunna neuronal kunnawa a wasu sassan kwakwalwa ba.
Saboda haka, hotuna daga yankunan Cg1 da amygdala (CeA da BLA) an yi amfani da su ta hanyar lambobi.Daga 400DCkyamara da software na mosaic don Windows, sigar 1.4 (Tucsen) ，Yankin hoton da aka samu shine 1.698 mm2 (1.304 mm x1.302 mm).
Hoton 1 Cue-induced sake dawo da cocaine ko neman tabar heroin yana da alaƙa da shigar da Fos a cikin PL, amma ba a cikin sauran wuraren cortical prefrontal (Cg1 da IL), kuma ba a cikin striatum ko amygdala ba.
(a) Yawan Fos-positive nuclei/mm2 (ma'anar ± SEM) a cikin mPFC (Cg1, PL, da IL)
(b) dorsal striatum (tsakiyar tsaka-tsaki da na gefe), ƙananan ƙwayoyin cuta (core da harsashi subregions), da amygdala (CeA da BLA subregions) don babu alamomi (n = 6-7), cocaine cue (n = 6), da heroin cue (n = 6). *p <0.05 dangane da ƙungiyar ba da alama. An dauki hotuna na kowane yanki na kwakwalwa daga wuraren da akwatunan baƙar fata na waje suka nuna akan tsarin tsarin sashin coronal. Takamammen wuraren da aka yi amfani da su don ƙididdige ƙwayoyin Fospositive Nuclei ana nuna su ta hanyar rufi masu launi.
(c) Hotunan wakilai na Fospositive nuclei a cikin PL da IL cortex.
Binciken fasahar hoto
Daga 400DCan yi amfani da kyamara don ɗaukar hotuna na yankuna daban-daban a cikin kwakwalwa a cikin wannan gwaji. Hakanan ya nuna kyakkyawan ikon ɗaukar hoto a cikin ƙaramin haske, wanda zai iya rage lokacin fallasa yadda ya kamata. Girman pixel na 6.5μm zai iya dacewa daidai da na'urar hangen nesa, kuma yana ɗaya daga cikin ƙananan kyamarori na kimiyya masu launi a kasuwa. Sakamakon gwajin kuma zai taimaka wajen nazarin shaye-shayen dan Adam na tabar heroin da hodar iblis.
Madogararsa Madogararsa
Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bossert, JM, Shaham, Y., & Hope, BT (2019). Prelimbic cortex yanki ne na kwakwalwa gama gari da aka kunna yayin da aka haifar da sake dawo da hodar iblis da tabar heroin da ke nema a cikin tsarin bera mai sarrafa kansa. Jaridar Turai na Neuroscience, 49 (2), 165-178.
