22/03/04ʻĀpana
Nui nā haʻawina preclinical i nānā i ka hoʻihoʻi hou ʻana i ka heroin a me ka cocaine e ʻimi ana i nā ʻano holoholona, akā ʻo ka hapa nui o kēia mau haʻawina i nānā i hoʻokahi lāʻau i ka manawa. I loko o nā mea hoʻohui kanaka, akā naʻe, hoʻohana maʻamau ka polydrug i ka cocaine a me ka heroin. Ua hoʻohana mākou i ka polydrug self-administration relapse model i nā ʻiole e hoʻoholo ai i nā mea like a me nā ʻokoʻa o nā wahi o ka lolo i hana ʻia i ka wā o ka cue-induced reinstatement o heroin a me ka ʻimi cocaine. ʻO ka holoʻokoʻa, hōʻike nā hopena he wahi lolo maʻamau ka PL i komo i ka heroin a me ka cocaine e ʻimi ana i ka wā o ka polydrug;
No laila, ua kiʻi ʻia nā kiʻi mai Cg1 a me nā ʻāpana amygdala (CeA a me BLA) me ka hoʻohana ʻana i kahiDhyana 400DCkamera a me ka polokalamu mosaic no Windows, version 1.4 (Tucsen)， ʻO ka wahi kiʻi i loaʻa he 1.698 mm2 (1.304 mm x1.302 mm).
Fig 1 ʻO ka hoʻihoʻi hou ʻana o ka cocaine a i ʻole ka ʻimi heroin e pili ana me Fos induction i ka PL, akā ʻaʻole ma nā wahi cortical prefrontal (Cg1 a me IL), ʻaʻole i ka striatum a i ʻole amygdala.
(a) Ka helu o Fos-positive nuclei/mm2 (mean ± SEM) ma mPFC (Cg1,PL, a me IL subregions),
(b) dorsal striatum (medial and lateral subregions), nucleus accumbens (core and shell subregions), a me amygdala (CeA a me BLA subregions) no ka ʻole cues (n = 6-7), cocaine cue (n = 6), a me heroin cue (n = 6) pūʻulu. *p <0.05 pili i ka hui ʻike ʻole. Ua kiʻi ʻia nā kiʻi no kēlā me kēia ʻāpana lolo mai nā wahi i hōʻike ʻia e nā pahu ʻeleʻele o waho ma nā schematics ʻāpana coronal. Hōʻike ʻia nā wahi hoʻohālike kikoʻī i hoʻohana ʻia no ka helu ʻana i nā nuclei Fospositive e nā uhi kala.
(c) Nā kiʻi ʻelele o Fospositive nuclei ma PL a me IL cortex.
ʻIkepili o ka ʻenehana kiʻi
Dhyana 400DCua hoʻohana ʻia ka pahupaʻikiʻi e kiʻi i nā kiʻi o nā ʻāpana like ʻole o ka lolo i kēia hoʻokolohua. Ua hōʻike pū ʻia ka hiki ke kiʻi kiʻi maikaʻi loa i ke kaiapuni haʻahaʻa haʻahaʻa, hiki ke hoʻopōkole pono i ka manawa hōʻike. Hiki ke hoʻohālikelike ka nui pixel o 6.5μm i ka microscope, a ʻo ia kekahi o nā kāmela ʻepekema kala liʻiliʻi ma ka mākeke. E kōkua pū nā hopena o ka hoʻokolohua i ka hoʻopaʻa ʻana o ke kanaka i ka heroin a me ka cocaine.
Kumu kuhikuhi
Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bosser, JM, Shaham, Y., & Hope, BT (2019). ʻO ka prelimbic cortex kahi wahi lolo maʻamau i hoʻolaʻa ʻia i ka wā o ka cue-induced reinstatement o ka cocaine a me ka heroin e ʻimi ana i kahi ʻano ʻiole polydrug self-administration. ʻO ka nūpepa ʻEulopa o ka neuroscience, 49 (2), 165-178.
