22.03.04 NwsAbstract
Ntau cov kev tshawb fawb preclinical tau tshuaj xyuas qhov kev rov ua rau tus neeg mob ntshav qab zib thiab cov yeeb tshuaj xas nrhiav hauv cov qauv tsiaj, tab sis feem ntau ntawm cov kev tshawb fawb no tau tshuaj xyuas ib qho tshuaj ib zaug xwb. Hauv tib neeg kev quav yeeb quav tshuaj, txawm li cas los xij, polydrug siv yeeb tshuaj yeeb thiab tshuaj yeeb dawb yog ntau. Peb siv polydrug tus kheej tswj tus kheej tus qauv rov ua dua tshiab hauv cov nas los txiav txim siab qhov sib xws thiab qhov sib txawv hauv cov cheeb tsam hauv lub hlwb tau qhib thaum lub sij hawm cue-induced rov qab los ntawm heroin thiab cocaine nrhiav. Zuag qhia tag nrho, cov txiaj ntsig tau qhia tias PL tej zaum yuav yog ib cheeb tsam hauv lub hlwb koom nrog hauv ob qho tib si heroin thiab cocaine nrhiav thaum siv polydrug; tsis muaj qhov tseem ceeb cueinduced neuronal activation tau pom nyob rau hauv lwm qhov chaw ntawm lub hlwb.
Yog li ntawd, cov duab los ntawm Cg1 thiab amygdala cheeb tsam (CeA thiab BLA) tau raug ntes digitally siv lubDhyana 400 DClub koob yees duab thiab mosaic software rau lub qhov rais, version 1.4 (Tucsen), qhov chaw imaging tau yog 1.698 mm2 (1.304 mm x 1.302 mm).
Daim duab 1 Cue-induced reinstatement ntawm cocaine los yog heroin nrhiav yog txuam nrog Fos induction nyob rau hauv lub PL, tab sis tsis nyob rau hauv lwm yam prefrontal cortical cheeb tsam (Cg1 thiab IL), thiab tsis nyob rau hauv lub striatum los yog amygdala.
(a) Tus naj npawb ntawm Fos-positive nuclei / mm2 (txhais tau tias ± SEM) hauv mPFC (Cg1, PL, thiab IL subregions),
(b) dorsal striatum (medial thiab lateral subregions), nucleus accumbens (core thiab plhaub subregions), thiab amygdala (CeA thiab BLA subregions) rau tsis muaj cues (n = 6–7), cocaine cue (n = 6), thiab heroin cue (n = 6) pawg. * p <0.05 txheeb ze rau pawg tsis muaj cues. Cov duab rau txhua cheeb tsam ntawm lub paj hlwb raug ntes los ntawm cov cheeb tsam uas qhia los ntawm cov thawv dub sab nraud ntawm lub coronal seem schematics. Cov chaw piv txwv tshwj xeeb uas siv rau kev ntsuas Fospositive nuclei yog qhia los ntawm cov xim pleev xim.
(c) Cov duab sawv cev ntawm Fospositive nuclei hauv PL thiab IL cortex.
Kev tsom xam ntawm kev siv technology
Dhyana 400 DCLub koob yees duab tau siv los ntes cov duab ntawm thaj chaw sib txawv hauv lub hlwb hauv qhov kev sim no. Nws kuj tau pom muaj peev xwm ua tau zoo tshaj plaws hauv qhov chaw teeb pom kev tsawg, uas tuaj yeem txo lub sijhawm raug. Lub pixel loj ntawm 6.5μm tuaj yeem ua tau zoo nrog lub tshuab tsom, thiab nws yog ib qho ntawm ob peb lub koob yees duab kev tshawb fawb xim ntawm lub khw. Cov txiaj ntsig ntawm qhov kev sim no tseem yuav pab tau rau hauv kev tshawb fawb txog tib neeg kev quav yeeb tshuaj yeeb dawb thiab yeeb dawb.
Kev siv qhov chaw
Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bossert, JM, Shaham, Y., & Hope, BT (2019). Prelimbic cortex yog ib qho chaw hauv lub hlwb tau qhib thaum lub sij hawm cue-induced reinstatement ntawm cocaine thiab heroin nrhiav nyob rau hauv ib tug polydrug tus kheej tswj nas qauv. European Journal of neuroscience, 49(2), 165–178.
