22/03/04Abstract
Ọtụtụ ọmụmụ ihe nyocha nyochara nlọghachi azụ nke heroin na cocaine na-achọ ụdị anụmanụ, mana ọtụtụ n'ime ọmụmụ ihe ndị a nyochara naanị otu ọgwụ n'otu oge. Otú ọ dị, n'ime ndị mmadụ riri ahụ, ịṅụ ọgwụ ọjọọ nke cocaine na heroin na-emekarị. Anyị jiri ihe atụ nlọghachị onwe nke polydrug n'ime oke iji chọpụta myirịta na ọdịiche dị na mpaghara ụbụrụ na-arụ ọrụ n'oge iweghachite heroin na cocaine na-achọ. N'ozuzu, nsonaazụ ya na-egosi na PL nwere ike ịbụ mpaghara ụbụrụ nkịtị metụtara ma heroin na cocaine na-achọ n'oge a na-eji polydrug eme ihe, ọ dịghị mkpa ka a na-eme ka neuronal rụọ ọrụ na mpaghara ụbụrụ ndị ọzọ.
Ya mere, ihe onyonyo sitere na mpaghara Cg1 na amygdala (CeA na BLA) ka ejiri akara dijitalụ weghara.Dyana 400DCIgwefoto na ngwanrọ mosaic maka Windows, ụdị 1.4 (Tucsen) ， Mpaghara onyonyo enwetara bụ 1.698 mm2 (1.304 mm x1.302 mm).
Fig 1 Mweghachi nke cocaine ma ọ bụ heroin na-achọ bụ njikọ Fos induction na PL, mana ọ bụghị na mpaghara ndị ọzọ prefrontal cortical (Cg1 na IL), ọ bụghị na striatum ma ọ bụ amygdala.
(a) Ọnụọgụ nke Fos-positive nuclei/mm2 (pụtara ± SEM) na mPFC (Cg1, PL, na IL subregions),
(b) dorsal striatum (mpaghara na mpụta), nucleus accumbens (isi na shei subregions), na amygdala (CeA na BLA subregions) maka enweghị akara (n = 6-7), cocaine cue (n = 6), na heroin cue (n = 6) otu. *p <0.05 n'ihe gbasara otu enweghị akara. Edere onyonyo maka mpaghara ụbụrụ ọ bụla site na mpaghara igbe ojii dị n'elu schematics nke akụkụ coronal gosipụtara. Ebe nlere anya akọwapụtara nke a na-eji maka ịkọwapụta nuclei Fospositive bụ ihe mkpuchi agba agba na-egosi.
(c) Foto ndị nnọchianya nke Fospositive nuclei na PL na IL cortex.
Nyocha nke teknụzụ onyonyo
Dyana 400DCejiri igwefoto were weghara onyonyo mpaghara dị iche iche na ụbụrụ na nnwale a. O gosikwara ikike ịse foto dị mma na gburugburu ọkụ dị ala, nke nwere ike belata oge mkpughe nke ọma. Nha pikselụ nke 6.5μm nwere ike dakọtara nke ọma na microscope, ọ bụkwa otu n'ime igwefoto sayensị agba ole na ole na ahịa. Nsonaazụ nke nnwale ahụ ga-enyekwara aka n'ịmụta ihe riri ahụ mmadụ na heroin na cocaine.
Isi mmalite ntụaka
Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bossert, JM, Shaham, Y., & Hope, BT (2019). Prelimbic cortex bụ mpaghara ụbụrụ nkịtị na-arụ ọrụ n'oge iweghachite cocaine na heroin na-achọ ụdị oke nchịkwa nchịkwa nke polydrug. Akwụkwọ akụkọ European nke Neuroscience, 49 (2), 165-178.
