22/03/04Ndemanga
Kafukufuku wambiri wachipatala adafufuza kuti abwererenso ku heroin ndi cocaine kufunafuna nyama, koma ambiri mwa maphunzirowa amafufuza mankhwala amodzi panthawi imodzi. Mwa anthu omwerekera, komabe, kugwiritsa ntchito mankhwala osokoneza bongo a cocaine ndi heroin ndikofala. Tidagwiritsa ntchito mtundu wodzilamulira wodzilamulira wa polydrug mu makoswe kuti tidziwe kufanana ndi kusiyana kwa madera aubongo omwe adakhazikitsidwa panthawi yobwezeretsanso heroin ndi cocaine kufunafuna. Ponseponse, zotsatira zake zikuwonetsa kuti PL ikhoza kukhala gawo laubongo lomwe limakhudzidwa ndi heroin ndi cocaine kufunafuna pakugwiritsa ntchito mankhwala ozunguza bongo; palibe chofunikira kwambiri choyambitsa neuronal activation chomwe chinawonedwa m'malo ena aubongo.
Chifukwa chake, zithunzi zochokera kumadera a Cg1 ndi amygdala (CeA ndi BLA) zidajambulidwa ndi digito pogwiritsa ntchitoDhyana 400DCkamera ndi zithunzi mapulogalamu Windows, Baibulo 1.4 (Tucsen)，Malo ojambulira analandira anali 1.698 mm2 (1.304 mamilimita x1.302 mm).
Chithunzi cha 1 Kubwezeretsanso kwa cocaine kapena kufunafuna heroin kumalumikizidwa ndi kulowetsedwa kwa Fos mu PL, koma osati m'malo ena a prefrontal cortical (Cg1 ndi IL), osati mu striatum kapena amygdala.
(a) Chiwerengero cha Fos-positive nuclei/mm2 (kutanthauza ± SEM) mu mPFC (Cg1,PL, and IL subregions),
(b) dorsal striatum (magawo apakatikati ndi ozungulira), ma nucleus accumbens (magawo apakati ndi zipolopolo), ndi amygdala (magawo a CeA ndi BLA) chifukwa chosagwirizana (n = 6-7), cocaine cue (n = 6), ndi magulu a heroin cue (n = 6). *p <0.05 wachibale ndi gulu la no cues. Zithunzi za dera lililonse laubongo zidajambulidwa kuchokera kumadera omwe akuwonetsedwa ndi mabokosi akunja akuda pamagawo a coronal schematics. Magawo achitsanzo omwe amagwiritsidwa ntchito poyesa ma Fospositive nuclei amawonetsedwa ndi zokutira zamitundu.
(c) Zithunzi zoyimira za Fospositive nuclei mu PL ndi IL cortex.
Kusanthula kwaukadaulo wojambula zithunzi
Dhyana 400DCkamera idagwiritsidwa ntchito kujambula zithunzi za zigawo zosiyanasiyana muubongo pakuyesaku. Inawonetsanso luso lojambula bwino kwambiri pamalo opepuka, omwe amatha kufupikitsa nthawi yowonekera. Kukula kwa pixel kwa 6.5μm kumatha kufanana bwino ndi maikulosikopu, ndipo ndi imodzi mwamakamera ochepa asayansi amitundu pamsika. Zotsatira za kuyesaku zithandizanso pakuphunzira za kusuta kwa heroin ndi cocaine.
Gwero lolozera
Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bossert, JM, Shaham, Y., & Hope, BT (2019). Prelimbic cortex ndi gawo laubongo lomwe limagwiritsidwa ntchito pobwezeretsanso cocaine ndi heroin pofunafuna makoswe a polydrug self-administration rat. European Journal of neuroscience, 49 (2), 165-178.
