22/03/04Abstract
Uphononongo oluninzi lwangaphambi kweklinikhi luvavanye ukuphinda ubuyele kwi-heroin kunye ne-cocaine kwiimodeli zezilwanyana, kodwa uninzi lwezi zifundo luvavanye ichiza elinye kuphela ngexesha. Kubantu abakhotyokisiweyo, nangona kunjalo, ukusetyenziswa kwe-polydrug ye-cocaine kunye ne-heroin kuyinto eqhelekileyo. Sisebenzise imodeli yokubuyisela i-polydrug self-administration kwiimpuku ukumisela ukufana kunye nomahluko kwiindawo zobuchopho ezenziwa ngexesha lokubuyiselwa kwe-heroin kunye ne-cocaine. Ngokubanzi, iziphumo zibonisa ukuba i-PL inokuba yindawo yengqondo eqhelekileyo ebandakanyekayo kuzo zombini i-heroin kunye ne-cocaine ifuna ngexesha lokusetyenziswa kwe-polydrug; akukho nto ibalulekileyo ye-cueinduced neuronal activation yabonwa kwezinye iindawo zobuchopho.
Ke ngoko, imifanekiso evela kwimimandla yeCg1 kunye ne-amygdala (CeA kunye ne-BLA) yabanjwa ngedijithali kusetyenziswaDhyana 400DCikhamera kunye nesoftware yemosaic yeWindows, uguqulelo 1.4 (Tucsen)，Indawo yomfanekiso efunyenweyo yayiyi-1.698 mm2 (1.304 mm x1.302 mm).
Umzobo we-1 Ukubuyiselwa kwe-cocaine okanye i-heroin ekufuneni inxulunyaniswa nokungeniswa kwe-Fos kwi-PL, kodwa hayi kwezinye iindawo zecortical yangaphambili (Cg1 kunye ne-IL), kwaye hayi kwi-striatum okanye i-amygdala.
(a) Inani le-Fos-positive nuclei/mm2 (ithetha ± SEM) kwi-mPFC (Cg1,PL, kunye ne-IL subregimens),
(b) i-dorsal striatum (i-medial kunye ne-lateral subregions), i-nucleus accumbens (i-core kunye ne-shell subregions), kunye ne-amygdala (i-CeA kunye ne-BLA subregions) ye-no cues (n = 6-7), i-cocaine cue (n = 6), kunye ne-heroin cue (n = 6) amaqela. *p <0.05 ngokunxulumene neqela elingekhoyo. Imifanekiso yommandla ngamnye wobuchopho ithathwe kwiindawo eziboniswe ziibhokisi ezimnyama ezingaphandle kwischematics yecandelo le-coronal. Iindawo ezithile zesampulu ezisetyenziselwa ukulinganisa i-Fospositive nuclei zibonakaliswe ngokugqithwa kombala.
(c) Imifanekiso emele i-Fospositive nuclei kwi-PL kunye ne-IL cortex.
Uhlalutyo lweteknoloji yokucinga
Dhyana 400DCIkhamera isetyenziselwe ukuthatha imifanekiso yemimandla eyahlukeneyo ebuchotsheni kolu vavanyo. Ikwabonise isakhono esihle sokucinga kwindawo yokukhanya okuphantsi, enokunciphisa ngokufanelekileyo ixesha lokuvezwa. Ubungakanani bepikseli ye-6.5μm bunokuhambelana ngokugqibeleleyo ne-microscope, kwaye yenye yeekhamera ezimbalwa zesayensi ezinombala kwimarike. Iziphumo zovavanyo ziya kunceda nasekufundeni ukuba likhoboka le-heroin kunye ne-cocaine.
Umthombo weReferensi
Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bossert, JM, Shaham, Y., & Hope, BT (2019). I-Prelimbic cortex yindawo yobuchopho eqhelekileyo eyenziwe yasebenza ngexesha lokubuyiselwa kwe-cocaine kunye ne-heroin ekufuneni imodeli yegundane yokulawula i-polydrug. Ijenali yaseYurophu ye-neuroscience, i-49 (2), i-165-178.
