22/03/04Abstract
Ucwaningo oluningi lwangaphambi komtholampilo luhlole ukuphindelwa kabusha okudalwe yi-cue ku-heroin ne-cocaine ekufuneni imodeli yezilwane, kodwa iningi lalezi zifundo lihlole umuthi owodwa kuphela ngesikhathi. Nokho, emiluthweni yabantu, ukusetshenziswa kwe-polydrug ye-cocaine ne-heroin kuvamile. Sisebenzise imodeli ye-polydrug self-administration relapse kumagundane ukuze sinqume ukufana nomehluko ezindaweni zobuchopho ezenziwe zasebenza ngesikhathi sokubuyiselwa okubangelwa i-heroin ne-cocaine. Sekukonke, imiphumela ibonisa ukuthi i-PL ingase ibe indawo evamile yobuchopho ehilelekile kukho kokubili ukufuna i-heroin ne-cocaine ngesikhathi sokusebenzisa i-polydrug; akukho ukuqaliswa okubalulekile kwe-neuronal okucatshangelwe okubonwe kwezinye izindawo zobuchopho.
Ngakho-ke, izithombe ezivela ezifundeni ze-Cg1 kanye ne-amygdala (i-CeA ne-BLA) zathwetshulwa ngedijithali kusetshenziswa i-I-Dhyana 400DCisofthiwe yekhamera ne-mosaic ye-Windows, inguqulo 1.4 (Tucsen)，Indawo yokuthwebula etholiwe ingu-1.698 mm2 (1.304 mm x1.302 mm).
I-Fig 1 Ukubuyiselwa okubangelwa i-Cue-okufunwa kwe-cocaine noma i-heroin kuhlotshaniswa nokungeniswa kwe-Fos ku-PL, kodwa hhayi kwezinye izindawo ze-prefrontal cortical (Cg1 ne-IL), futhi hhayi ku-striatum noma i-amygdala.
(a) Inombolo ye-Fos-positive nuclei/mm2 (isho ± SEM) ku-mPFC (izifunda ezingaphansi kwe-Cg1,PL, ne-IL),
(b) i-dorsal striatum (izifunda ezimaphakathi nezasemuva), i-nucleus accumbens (izindawo ezingaphansi negobolondo), kanye ne-amygdala (izifunda ze-CeA ne-BLA) zokungasho lutho (n = 6–7), i-cocaine cue (n = 6), kanye namaqembu e-heroin cue (n = 6). *p <0.05 ngokuhlobene neqembu le-no cues. Izithombe zesifunda sobuchopho ngasinye zithathwe ezindaweni ezikhonjiswe amabhokisi amnyama angaphandle ku-schematics yesigaba se-coronal. Izindawo ezithile zamasampula ezisetshenziselwa ukulinganisa i-Fospositive nuclei ziboniswa yimbondela enemibala.
(c) Izithombe ezimele ze-Fospositive nuclei ku-PL kanye ne-IL cortex.
Ukuhlaziywa kobuchwepheshe bezithombe
I-Dhyana 400DCikhamera isetshenziswe ukuthwebula izithombe zezifunda ezahlukahlukene ebuchosheni kulokhu kuhlolwa. Iphinde yabonisa ikhono elihle kakhulu lokucabanga endaweni yokukhanya okuphansi, engafinyeza ngempumelelo isikhathi sokuchayeka. Usayizi wephikseli ongu-6.5μm ungafanelana kahle nesibonakhulu, futhi ungelinye lamakhamera ambalwa esayensi anombala emakethe. Imiphumela yokuhlolwa izosiza futhi ocwaningweni lokuluthwa komuntu i-heroin ne-cocaine.
Umthombo wesithenjwa
U-Rubio, FJ, Quintana-Feliciano, R., Warren, BL, Li, X., Witonsky, K., Valle, F., Selvam, PV, Caprioli, D., Venniro, M., Bossert, JM, Shaham, Y., & Hope, BT (2019). I-Prelimbic cortex iyindawo yobuchopho evamile eyenziwa yasebenza ngesikhathi sokubuyiselwa okubangelwa i-cocaine ne-heroin kumodeli yegundane elizilawulayo le-polydrug. I-European Journal of Neuroscience, 49 (2), 165-178.
