23/10/10Nguva inononoka & kusanganisa (TDI) inzira yekutora mufananidzo yakavakirwa pamusimboti wekutarisa mutsara, apo nhevedzano yeimwe-dimensional mifananidzo inotorwa kuti ibudise mufananidzo nekutarisa nguva yemuenzaniso kufamba uye mufananidzo chidimbu kutorwa nekuita. Kunyangwe tekinoroji iyi yanga iripo kwemakumi emakore, yagara ichibatanidzwa neakaderera-sensitivity application, senge webhu kuongorora.
Chizvarwa chitsva chemakamera akabatanidza kunzwisiswa kwesCMOS nekumhanya kweTDI kupa mufananidzo kutorwa kwemhando yakaenzana kune scanner yenzvimbo asi nekugona kwemirairo yehukuru inokurumidza kubuda. Izvi zvinonyanya kuoneka mumamiriro ezvinhu apo kufungidzira kwemasampuli makuru mumamiriro ekuderera kwechiedza kunodiwa. Mune ino tekinoroji chinyorwa, tinonongedza mashandiro anoita TDI scanning, uye toenzanisa iyo nguva yekutora mufananidzo kune inofananidzwa yakakura nzvimbo yekuvheneka nzira, mataira & musono imaging.
Kubva pakuvheneka mutsara kuenda kuTDI
Line scan imaging inzira yekufungidzira inoshandisa mutsara mumwe chete wemapikisi (unonzi koramu, kana dariro) kutora chidimbu chemufananidzo apo sample iri kufamba. Uchishandisa nzira dzemagetsi dzinokonzeresa, 'chidimbu' chimwe chemufananidzo chinotorwa sezvo sampuli ichipfuura sensor. Nekuyera iyo kamera inokonzeresa chiyero chekutora mufananidzo pamwe nekufamba kwemuenzaniso uye kushandisa furemu grabber kutora iyi mifananidzo, inogona kubatanidza kuti ivakezve mufananidzo.
TDI imaging inovaka pane iyi musimboti wekutorwa kwemufananidzo wemuenzaniso, zvisinei, inoshandisa nhanho dzakawanda kuwedzera huwandu hwemafotoelectrons akatorwa. Sezvo muenzaniso uchipfuura nhanho imwe neimwe, mamwe mashoko anounganidzwa uye akawedzerwa kune aripo photoelectrons akatorwa nematanho ekutanga uye akashongedzwa nenzira yakafanana kune yeCCD zvishandiso. Sezvo muenzaniso uchipfuura padanho rekupedzisira, maphotoelectrons akaunganidzwa anotumirwa kukuverenga, uye chiratidzo chakabatanidzwa mukati mechikamu chinoshandiswa kugadzira chidimbu chemufananidzo. Mumufananidzo 1, kutorwa kwemufananidzo pamudziyo une makoramu mashanu eTDI (matanho) anoratidzwa.
Mufananidzo 1: muenzaniso wehupenyu wekutora mufananidzo uchishandisa TDI tekinoroji. Sample (yebhuruu T) inopfuudzwa pamudziyo wekutora mufananidzo weTDI (mukoramu wemapikisesi mashanu, nhanho shanu dzeTDI), uye maphotoelectrons anotorwa padanho rega rega owedzerwa padanho rechiratidzo. Chiverengero chinoshandura ichi kuita mufananidzo wedhijitari.
1a: Mufananidzo (T yebhuruu) inounzwa pachikuva; iyo T iri kufamba sezvakaratidzwa pamudziyo.
1b: Sezvo T ichipfuura nhanho yekutanga, kamera yeTDI inotangwa kuti igamuchire maphotoelectrons anotorwa nemapikseli paanorova nhanho yekutanga paTDI sensor. Koramu yega yega ine nhevedzano yepixels inotora maphotoelectrons ega.
1c: Aya maphotoelectrons akabatwa anokandwa kusvika padanho rechipiri, apo mbiru yega yega inosundira nhanho yayo yechiratidzo kusvika padanho rinotevera.
1d: Nekufamba kwenguva nekufamba kweyemuenzaniso imwe-pixel chinhambwe, seti yechipiri yemafotoelectrons inotorwa pachikuva chechipiri, yowedzerwa kune yakambotorwa, ichiwedzera chiratidzo. Muchikamu chekutanga, seti nyowani yemafotoelectrons inotorwa, inoenderana nechidimbu chinotevera chekutora mufananidzo.
1e: Maitiro ekutora mifananidzo anotsanangurwa padanho 1d anodzokororwa sezvo mufananidzo unofamba uchipfuura sensor. Izvi zvinovaka chiratidzo kubva kumafotoelectrons kubva kumatanho. Chiratidzo chinopfuudzwa mukuverenga, icho chinoshandura chiratidzo chephotoelectron kuita kuverenga kwedhijitari.
1f: Kuverenga kwedhijitari kunoratidzwa sekoramu yemufananidzo nekoramu. Izvi zvinobvumira kugadzirwa patsva kwechifananidzo.
Sezvo muchina weTDI uchikwanisa kupfuudza maphotoelectrons panguva imwe chete kubva pane chimwe chinhanho kuenda kune chinotevera, uye kutora maphotoelectrons matsva kubva padanho rekutanga apo sampuli iri kufamba, mufananidzo wacho unogona kuperezeka muhuwandu hwemitsara yakatorwa. Iwo matrigger rates, anotaridza huwandu hwenguva dzekutorwa kwemufananidzo (fig 1a) inoitika, inogona kunge iri pahurongwa hwemazana ekHz.
Mumuenzaniso weMufananidzo 2, siraidhi ye 29 x 17 mm maikorosikopu yakatorwa mumasekonzi gumi nechidimbu pachishandiswa 5 µm pixel TDI kamera. Kunyangwe pamazinga akakosha e zoom, nhanho yekusviba ishoma. Izvi zvinomiririra kufambira mberi kukuru pazvizvarwa zvakapfuura zveiyi tekinoroji.
Kuti uwane rumwe ruzivo, Tafura 1 inoratidza inomiririra nguva yekufungidzira kune akateedzana saizi saizi akajairwa pa10, 20, uye 40 x zoom.
Mufananidzo 2: Mufananidzo wemuenzaniso wefluorescent wakatorwa uchishandisa Tucsen 9kTDI. Exposure 10 ms, nguva yekutora 10.1 s.
Tafura 1: Matrix yenguva yekubata yeakasiyana saizi saizi (masekondi) uchishandisa Tucsen 9kTDI kamera paZaber MVR series motorized stage pa10, 20, uye 40 x for 1 & 10 ms exposure time.
Area scan imaging
Nzvimbo yekuongorora yekufungidzira mumakamera eCMOS inosanganisira kutora mufananidzo wese panguva imwe chete uchishandisa 2-dimensional array yemapikisi. Pixel yega yega inotora mwenje, ichichishandura kuita masaini emagetsi kuti agadziriswe nekukurumidza uye kugadzira mufananidzo wakazara nepamusoro resolution uye nekumhanya. Saizi yemufananidzo unogona kutorwa mukuratidzwa kamwe chete inodzorwa nehukuru hwepixel, magnification, uye nhamba yemapikiseli muhurongwa, pa (1)
Kune yakajairwa array, iyo ndima yekuona inopiwa ne (2)
Mumamiriro ezvinhu apo sampuli yakakura zvikuru kune munda wekuona kwekamera, mufananidzo unogona kugadzirwa nekuparadzanisa chifananidzo mugridhi yemifananidzo yehukuru hwemunda wekuona. Kutorwa kwemifananidzo iyi kunotevera muenzaniso, uko nhanho ichaenda kune chinzvimbo pane grid, nhanho inogadzika, uye ipapo mufananidzo uchatorwa. Mukutenderedza shutter makamera, pane imwe nguva yekumirira apo shutter inotenderera. Iyi mifananidzo inogona kutorwa nekufambisa chinzvimbo chekamera uye nekuibatanidza pamwechete. Mufananidzo 3 unoratidza mufananidzo muhombe wesero remunhu riri pasi pefluorescence microscopy rakagadzirwa nekusonanidza pamwe 16 mifananidzo midiki.
Mufananidzo 3: Slide yesero remunhu riri kutorwa nenharaunda scanner kamera uchishandisa mataira & stitch imaging.
Kazhinji, kugadzirisa zvakadzama kunoda mamwe mapikicha kuti agadzirwe uye asonanidzwe pamwechete nenzira iyi. Imwe mhinduro kune izvi ndeyekushandisayakakura fomati kamera scanning, iyo ine ma sensors akakura ane yakakwira pixel count, tandem ine specialized optics, inobvumira huwandu hukuru hweruzivo kutorwa.
Kuenzanisa pakati peTDI uye nzvimbo yekuongorora (Tile & Stitch)
Kune yakakura-nzvimbo yekuongorora yemasampuli, zvese Tile & Stitch uye TDI scanning imhinduro dzakakodzera, zvisinei nekusarudza nzira yakanakisa, zvinokwanisika kudzikisa nguva inodiwa kuongorora sampuli zvakanyanya. Ino nguva yekuchengetedza inogadzirwa nekugona kweTDI scanning kutora sampuli inofamba; kubvisa kunonoka kwakabatana nekugadzirisa nhanho uye rolling shutter nguva yakabatana ne tile & stitch imaging.
Mufananidzo 4 unofananidza kumira (girinhi) uye mafambiro (mitsetse mitema) inodiwa kutora mufananidzo wesero remunhu mune ese matile & stitch (kuruboshwe), uye TDI (kurudyi) kuongorora. Nekubvisa kudiwa kwekumisa uye kugadzirisa chifananidzo muTDI imaging, zvinokwanisika kuderedza nguva yekufungidzira zvakanyanya, kupa nguva yekuratidzira yakaderera <100 ms.
Tafura 2 inoratidza muenzaniso wakashanda wekutarisa pakati pe9k TDI uye yakajairwa sCMOS kamera.
Mufananidzo 4: A scanning motif yekutorwa kwesero remunhu pasi pefluorescence inoratidza tile uye musono (kuruboshwe) uye TDI imaging (kurudyi).
Tafura 2: Kuenzanisa kwenzvimbo scan uye TDI imaging ye 15 x 15 mm sampuli ine 10x chinangwa lens uye 10 ms exposure time.
Nepo TDI ichipa yakanakisa mukana wekuwedzera kukurumidza kwekutora mufananidzo, kune manuances pakushandiswa kweiyi tekinoroji. Nezvenguva dzepamusoro (> 100 ms), kukosha kwenguva yakarasika pakufamba uye kugadzirisa zvinhu zvenzvimbo yekuongorora kunoderedzwa maererano nenguva yekuratidzwa. Muzviitiko zvakadaro, nzvimbo scanning makamera anogona kupa yakaderedzwa scan nguva zvichienzaniswa neTDI imaging. Kuti uone kana TDI tekinoroji inogona kukupa mabhenefiti pane yako yazvino kuseta,taura nesuyekuenzanisa karukureta.
Mamwe maapplication
Mibvunzo mizhinji yekutsvagisa inoda ruzivo rwakawanda kupfuura mufananidzo mumwechete, senge multichannel kana multifocus mufananidzo kutora.
Multichannel imaging munharaunda scanner kamera inosanganisira kutora mifananidzo uchishandisa akawanda wavelengths panguva imwe chete. Aya machanera anowanzoenderana neakasiyana wavelengths echiedza, senge tsvuku, girini, uye bhuruu. Imwe neimwe chiteshi inotora chaiyo wavelength kana spectral ruzivo kubva panzvimbo. Iyo kamera inozobatanidza aya machanera kuti agadzire yakazara-ruvara kana multispectral mufananidzo, ichipa yakawedzera kutarisisa yechiitiko ine akasiyana spectral ruzivo. Munzvimbo yekuongorora makamera, izvi zvinowanikwa nekuratidzwa pachena, zvisinei, nekufungidzira kweTDI, splitter inogona kushandiswa kuparadzanisa sensor muzvikamu zvakawanda. Kupatsanura 9kTDI (45 mm) kuita 3 x 15.0 mm masensa kuchave kuchiri kuhombe pane standard sensor (6.5 µm pixel wide, 2048 pixels) hupamhi hwe13.3 mm. Uyezve, sezvo TDI ichingoda kuvhenekerwa pachikamu chemuenzaniso uri kufananidzwa, ma scans anogona kufambiswa nekukurumidza.
Imwe nharaunda iyo izvi zvingave zvakadaro iri mu-multi-focus imaging. Multifocus imaging munharaunda scanca kamera inosanganisira kutora mifananidzo yakawanda pamadaro akasiyana ekutarisa uye nekuisanganisa kuti igadzire mufananidzo unoumbwa nechiitiko chese mukutarisisa. Inogadzirisa madaro akasiyana-siyana muchiitiko nekuongorora uye nekubatanidza-in-focus matunhu kubva pamufananidzo wega wega, zvichikonzera kuratidzwa kwakadzama kwemufananidzo. Zvakare, nekushandisa asplitterkupatsanura iyo TDI sensor kuita maviri (22.5 mm), kana matatu (15.0 mm) zvidimbu, zvingave zvichikwanisika kuwana multifocus mufananidzo nekukurumidza kupfuura nharaunda scan yakaenzana. Kune yakakwira order multifocus (z stacks of 6 kana mukuru) zvisinei, area scan ingangoramba iriyo inokurumidza kufungidzira nzira.
Mhedziso
Ichi chinyorwa chetekinoroji chinotaridza mutsauko uripo pakati penzvimbo yekuongorora uye TDI tekinoroji yekutarisisa nzvimbo huru. Nekubatanidza mitsara yekuongorora uye sCMOS senitivity, TDI inowana nekukurumidza, yemhando yepamusoro yekufungidzira pasina kukanganisa, kupfuura echinyakare nharaunda scan nzira senge tile & musono. Ongorora zvakanakira kushandisa yedu online Calculator, uchifunga dzakasiyana fungidziro dzakatsanangurwa mugwaro iri. TDI inomira sechishandiso chine simba chekufungidzira kwakanaka chine mukana mukuru wekudzikisa nguva dzekufungidzira mune ese akajairwa uye epamusoro ekufungidzira maitiro.Kana iwe uchida kuona kana TDI kamera kana nharaunda scanner kamera inogona kuenzanisa application yako uye kugadzirisa yako nguva yekutora, taura nesu nhasi.
