22/03/03Abtract
Gabatarwar AuNRs da PPTT na iya shafar haɗin kai tsakanin cytoskeleton da tantanin halitta, don haka hana ƙaura gama gari na ƙwayoyin kansa. STORM ya kara lura da rushewar cytoskeleton na actin ta hanyar amfani da microscope na superresolution, yayin da aka yi amfani da wani microscope don yin rikodin hotunan DIC a cikin gwaje-gwajen haɓakawa (har zuwa 200x). Sakamako ya nuna cewa AuNRs da aka yi niyya da haɗin kai da hulɗar tantanin halitta na iya haifar da canje-canjen phosphorylation. Canje-canje a cikin ilimin halittarsu ko matakan magana, waɗanda sune mahimman abubuwan ɓangarorin cytoskeletal filaments da haɗin sel, kuma suna hana ƙaura.
Hoto 1 Kwatanta ƙudurin STORM (A) da kuma na al'ada mai kyalli microscopy imaging (B) don actin filaments.
Hoto 2 Shan kwayar salula, cytotoxicity da motsi akan jiyya na AuNRs. (AB) Bambanci
bambancin tsangwama (DIC) ƙananan hotuna na sel HeLa ba tare da (A) ba kuma tare da
AuNRs@RGD bayan 24h incubation (B). (C) Hoton DIC na AuNRs@RGD yana rarrabawa a wuraren mahaɗar tantanin halitta bayan 24h incubation.
Binciken fasahar hoto
Microscopy na al'ada fluorescence yana da wuya a karya ta hanyar iyakacin tsinkaye na gani, rashin iya lura da tasirin gabatarwar nanorods na zinariya da PPTT akan sel, ba tare da babban ƙuduri ba, ba shi yiwuwa a kwatanta su.Daya 95kuma400 BSIkyamarori ƙananan kyamarori ne guda biyu, masu ƙarfi da sassauƙa na sCMOS waɗanda suka dace da wannan aikace-aikacen. Kyamarar tana da nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan sun haɗa da yanayin ƙaramar ƙarar ƙarar CMS da babban yanayi mai ƙarfi. Backshot sCMOS ba wai kawai cimma daidaitaccen daidaitaccen adadin 95% ba, har ma yana da babban filin kallo kamar 2 ", Amsa mai faɗi na 200-1100nm yana da amfani don haɓaka haɓakar hankali da haɓakar kewayon manyan pixels 11um, wanda sauran kyamarorin sCMOS ba su da fa'idodi da yawa a cikin gajeren lokaci. tsawon lokaci ba tare da haifar da lalacewar hoto da bleaching na samfurori ba.
Madogararsa Madogararsa
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Zinariya Nanorod Photothermal Therapy Yana Canza Matsakanin Salon salula da Cibiyar Sadarwar Actin a Hana Hijirar Haɗin Ciwon Ciwon daji. ACS Nano. 2018 Satumba 25; 12 (9): 9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Agusta 27. PMID: 30118603; Saukewa: PMC6156989
