The introduction of AuNRs and PPTT can affect the connection between cytoskeleton and cell, thus inhibiting the collective migration of cancer cells. Disruption of the actin cytoskeleton was further observed by STORM using a superresolution microscope, while another microscope was used to record DIC images in magnification experiments (up to 200x). Results showed that integrin-targeted AuNRs and cell-cell interactions could trigger phosphorylation changes. Changes in their morphology or expression levels, which are key components of cytoskeletal filaments and cell connections, also inhibit tumor migration.
Fig. 1 Comparison of the resolution of STORM (A) and conventional fluorescence microscopy imaging (B) for actin filaments.
Fig. 2 Cellular uptake, cytotoxicity and motility upon AuNRs treatments. (A-B) Differential
interference contrast (DIC) microscopic images of HeLa cells without (A) and with
AuNRs@RGD after 24 h incubation (B). (C) DIC image of AuNRs@RGD distribute in the cell junction areas after 24 h incubation.
Analysis of imaging technology
Traditional fluorescence microscopy is difficult to break through the limit of optical diffraction, unable to observe the effect of the introduction of gold nanorods and PPTT on cells, without super resolution, it is impossible to image them. Dhyana 95 and 400BSI cameras are two compact, powerful and flexible sCMOS cameras that are ideal for this application. The cameras have a variety of different modes including CMS low noise readout mode and high dynamic mode. Backshot sCMOS not only achieve near-perfect 95% quantum efficiency, but also have large field of view such as 2", The wide spectral response of 200-1100nm is conducive to improving the sensitivity and dynamic range of 11um large pixels, which other sCMOS cameras do not have many advantages. Therefore, imaging can be performed at lower illumination intensity and shorter exposure time over a longer period of time without causing photodamage and photobleaching of samples.
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Gold Nanorod Photothermal Therapy Alters Cell Junctions and Actin Network in Inhibiting Cancer Cell Collective Migration. ACS Nano. 2018 Sep 25;12(9):9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Aug 27. PMID: 30118603; PMCID: PMC6156989