22/03/03ʻĀpana
Hiki i ka hoʻokomo ʻana o AuNRs a me PPTT ke hoʻopili i ka pilina ma waena o ka cytoskeleton a me ke kelepona, no laila ke kāohi nei i ka neʻe hui ʻana o nā pūnaʻi kanesa. Ua ʻike hou ʻia ka haunaele o ka cytoskeleton actin e STORM me ka hoʻohana ʻana i kahi microscope superresolution, aʻo kekahi microscope i hoʻohana ʻia e hoʻopaʻa i nā kiʻi DIC i nā hoʻokolohua hoʻonui (a hiki i 200x). Ua hōʻike ʻia nā hopena i hiki i ka integrin-targeted AuNRs a me nā pilina cell-cell ke hoʻoulu i nā loli phosphorylation. ʻO nā hoʻololi i kā lākou morphology a i ʻole pae hōʻike, ʻo ia nā ʻāpana koʻikoʻi o nā filament cytoskeletal a me nā pili pūnaewele, ke kāohi pū nei i ka neʻe ʻana o ka maʻi tumora.
Fig. 1 Hoʻohālikelike o ka hoʻonā o STORM (A) a me ka fluorescence microscopy imaging (B) no nā filament actin.
Fig. 2 Ka lawe ʻana i ke kelepona, cytotoxicity a me ka motility ma luna o nā lāʻau AuNRs. (AB) ʻokoʻa
interference contrast (DIC) kiʻi microscopic o nā pūnaewele HeLa me ka ʻole (A) a me
AuNRs@RGD ma hope o 24 h incubation (B). (C) DIC kiʻi o AuNRs@RGD puʻunaue i loko o ka cell junction wahi ma hope o 24 h incubation.
ʻIkepili o ka ʻenehana kiʻi
He mea paʻakikī ka microscopy fluorescence kuʻuna i ka palena o ka diffraction optical, ʻaʻole hiki ke nānā i ka hopena o ka hoʻokomo ʻana o nā nanorods gula a me PPTT ma nā pūnaewele, me ka ʻole o ka hoʻonā super, hiki ʻole ke kiʻi iā lākou.Dhyana 95a400BSIʻO nā kiʻi paʻi kiʻi ʻelua mau kāmeʻa sCMOS paʻa, ikaika a maʻalahi i kūpono no kēia noi. Loaʻa i nā kāmela nā ʻano ʻano like ʻole e like me ke ʻano heluhelu haʻahaʻa haʻahaʻa CMS a me ke ʻano hoʻoikaika kiʻekiʻe. ʻAʻole hiki i ka Backshot sCMOS ke hoʻokō wale i ka 95% quantum efficiency, akā loaʻa pū kekahi i ka nui o ka nānā ʻana e like me 2", ʻO ka pane spectral ākea o 200-1100nm he mea kūpono ia i ka hoʻomaikaʻi ʻana i ka naʻau a me ka dynamic range o 11um nui pixels, ʻaʻole i loaʻa i nā kāmela sCMOS ʻē aʻe nā pōmaikaʻi he nui. manawa me ka hoʻopōʻino ʻole ʻana i ka paʻi kiʻi a me ka paʻi kiʻi ʻana o nā laʻana.
Kumu kuhikuhi
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Hoʻololi ʻo Gold Nanorod Photothermal Therapy i nā hui pūnaewele a me Actin Network i ke kāohi ʻana i ka neʻe ʻana o ka maʻi maʻi. ACS Nano. 2018 Sep 25;12(9):9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Aug 27. PMID: 30118603; PMCID: PMC6156989
