22.03.2019 03.03 NwsAbstract
Kev taw qhia ntawm AuNRs thiab PPTT tuaj yeem cuam tshuam kev sib txuas ntawm cytoskeleton thiab cell, yog li inhibiting kev sib koom ua ke ntawm cov qog nqaij hlav cancer. Kev cuam tshuam ntawm cov actin cytoskeleton tau soj ntsuam ntxiv los ntawm STORM siv lub tshuab ntsuas superresolution, thaum lwm lub tshuab tsom iav tau siv los sau cov duab DIC hauv kev sim ua kom pom tseeb (txog 200x). Cov txiaj ntsig tau pom tias kev sib xyaw ua ke ntawm AuNRs thiab kev sib cuam tshuam ntawm tes ntawm tes tuaj yeem ua rau muaj kev hloov pauv phosphorylation. Kev hloov pauv hauv lawv cov morphology lossis kev qhia qib, uas yog cov khoom tseem ceeb ntawm cytoskeletal filaments thiab kev sib txuas ntawm tes, kuj inhibit qog migration.
Fig. 1 Kev sib piv ntawm qhov kev daws teeb meem ntawm STORM (A) thiab cov qauv fluorescence microscopy imaging (B) rau actin filaments.
Fig. 2 Cellular uptake, cytotoxicity thiab motility raws li kev kho mob AuNRs. (AB) Differential
interference contrast (DIC) microscopic dluab ntawm HeLa hlwb tsis muaj (A) thiab nrog
AuNRs@RGD tom qab 24 teev incubation (B). (C) DIC duab ntawm AuNRs@RGD faib rau hauv cov chaw sib txuas ntawm tes tom qab 24 teev incubation.
Kev tsom xam ntawm kev siv technology
Cov tshuaj fluorescence microscopy yog qhov nyuaj rau kev ua txhaum ntawm qhov txwv ntawm qhov muag pom, tsis tuaj yeem soj ntsuam cov txiaj ntsig ntawm kev qhia txog kub nanorods thiab PPTT ntawm cov hlwb, tsis muaj kev daws teeb meem super, nws tsis tuaj yeem ua duab rau lawv.Tshooj 95thiab400 BSIcov koob yees duab yog ob lub compact, haib thiab saj zawg zog sCMOS koob yees duab uas yog zoo tagnrho rau daim ntawv thov no. Cov koob yees duab muaj ntau hom kev sib txawv xws li CMS low noise readout mode thiab high dynamic mode. Backshot sCMOS tsis tsuas yog ua tiav ze-zoo meej 95% quantum efficiency, tab sis kuj muaj qhov loj ntawm kev pom xws li 2", Qhov dav spectral teb ntawm 200-1100nm yog qhov tsim nyog rau kev txhim kho qhov rhiab heev thiab dynamic ntau ntawm 11um loj pixels, uas lwm lub koob yees duab sCMOS luminous tsis muaj ntau qhov zoo ntawm kev ua tau zoo. Lub sij hawm raug luv dua nyob rau lub sijhawm ntev dua yam tsis ua rau photodamage thiab photobleaching ntawm cov qauv.
Kev siv qhov chaw
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Kub Nanorod Photothermal Therapy Alters Cell Junctions thiab Actin Network hauv Inhibiting Cancer Cell Collective Migration. ACS Nano. 2018 Sep 25; 12(9): 9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Aug 27. PMID: 30118603; PIB: PMC6156989
