22/03/03Abstract
Iwebata AuNRs na PPTT nwere ike imetụta njikọ dị n'etiti cytoskeleton na cell, si otú a na-egbochi mbugharị mkpokọta nke mkpụrụ ndụ kansa. STORM gara n'ihu hụ mmebi nke cytoskeleton actin site na iji microscope superresolution, ebe a na-eji microscope ọzọ dekọọ onyonyo DIC na nnwale mmụba (ruo 200x). Nsonaazụ gosiri na AuNR nke etinyebere na ntinye na mmekọrịta cell-cell nwere ike ịkpalite mgbanwe phosphorylation. Mgbanwe na ọdịdị ọdịdị ọdịdị ha ma ọ bụ ọkwa nkwupụta ha, nke bụ isi akụkụ nke filament cytoskeletal na njikọ cell, na-egbochikwa ịkwaga tumor.
Fig. 1 Ntụle nke mkpebi nke STORM (A) na ihe ngosi nke microscopy fluorescence (B) maka actin filaments.
Fig. 2 Mwelite cellular, cytotoxicity na motility na ọgwụgwọ AuNRs. (AB) dị iche iche
ọdịiche nnyonye anya (DIC) onyonyo onyonyo nke mkpụrụ ndụ HeLa na-enweghị (A) yana ya
AuNRs@RGD mgbe 24 h nnabata (B). (C) Onyonyo DIC nke AuNRs@RGD na-ekesa na mpaghara njikọ cell ka 24 h gachara.
Nyocha nke teknụzụ onyonyo
Omenala fluorescence microscopy siri ike imebi site na njedebe nke anya diffraction, enweghị ike ịhụ mmetụta nke iwebata gold nanorods na PPTT na sel, na-enweghị nnukwu mkpebi, ọ gaghị ekwe omume iji image ha.Dịna 95na400BSIIgwefoto bụ kọmpat abụọ, dị ike na nke na-agbanwe agbanwe nke sCMOS dị mma maka ngwa a. Igwefoto nwere ụdịdị dị iche iche dị iche iche gụnyere CMS obere mkpọtụ mkpọtụ na ọnọdụ dị elu. Backshot sCMOS ọ bụghị naanị na-enweta arụmọrụ zuru oke nke 95% zuru oke, kamakwa nwee nnukwu echiche dị ka 2”, nzaghachi dị iche iche nke 200-1100nm na-enyere aka melite nghọta na oke ike nke 11um nnukwu pikselụ, nke igwefoto sCMOS ndị ọzọ enweghị ike ịrụ ọrụ dị mkpụmkpụ na obere oge. ogologo oge na-enweghị ime ka foto mebiri na fotobleaching nke samples.
Isi mmalite ntụaka
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Gold Nanorod Photothermal Therapy na-agbanwe njikọ sel na Actin Network na-egbochi Mbugharị mkpokọta mkpụrụ ndụ kansa. ACS Nano. 2018 Sep 25;12 (9): 9279-9290 . doi: 10.1021/acsnano.8b04128. Epub 2018 Ọgọst 27. PMID: 30118603; PMCID: PMC6156989
