22/03/03Ndemanga
Kuyambitsidwa kwa AuNRs ndi PPTT kungakhudze kugwirizana pakati pa cytoskeleton ndi selo, motero kulepheretsa kusamuka pamodzi kwa maselo a khansa. Kusokoneza kwa actin cytoskeleton kunawonedwanso ndi STORM pogwiritsa ntchito maikulosikopu apamwamba kwambiri, pomwe maikulosikopu ina idagwiritsidwa ntchito kujambula zithunzi za DIC pakuyesa kukulitsa (mpaka 200x). Zotsatira zinawonetsa kuti ma AuNR omwe amatsata ma integrin ndi kuyanjana kwa ma cell kungayambitse kusintha kwa phosphorylation. Kusintha kwa ma morphology kapena mafotokozedwe awo, omwe ndi zigawo zazikulu za cytoskeletal filaments ndi kulumikizana kwa ma cell, zimalepheretsanso kusamuka kwa chotupa.
Mkuyu 1 Kuyerekeza kusamvana kwa STORM (A) ndi ochiritsira fluorescence microscopy kujambula (B) kwa actin filaments.
Chithunzi 2 Kutenga ma cell, cytotoxicity ndi motility pamankhwala a AuNRs. (AB) Zosiyana
Kusokoneza kusiyana (DIC) zithunzi zazing'ono za maselo a HeLa opanda (A) ndi
AuNRs@RGD pambuyo pa makulitsidwe a 24 h (B). (C) Chithunzi cha DIC cha AuNRs@RGD chigawidwe m'malo olumikizirana ma cell pambuyo pa kukulitsidwa kwa 24 h.
Kusanthula kwaukadaulo wojambula zithunzi
Traditional fluorescence microscopy ndizovuta kudutsa malire a kuwala diffraction, osatha kuona zotsatira za kumayambiriro nanorods golide ndi PPTT pa maselo, popanda wapamwamba kusamvana, ndi kosatheka fano iwo.Dyana 95ndiMtengo wa 400BSImakamera ndi makamera awiri ophatikizika, amphamvu komanso osinthika a sCMOS omwe ali abwino kugwiritsa ntchito pulogalamuyi. Makamera ali ndi mitundu yosiyanasiyana yosiyana siyana kuphatikizapo CMS low noise readout mode ndi high dynamic mode. Backshot sCMOS sikuti imangofikitsa pafupifupi 95% kuchuluka kwachulukidwe, komanso imakhala ndi mawonekedwe akulu ngati 2 ", Kuyankha kowoneka bwino kwa 200-1100nm kumathandizira kukulitsa chidwi komanso kusinthasintha kwa ma pixel akulu 11um, omwe makamera ena a sCMOS alibe maubwino ambiri. nthawi yayitali popanda kuchititsa photodamage ndi photobleaching ya zitsanzo.
Gwero lolozera
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Gold Nanorod Photothermal Therapy Alters Cell Junctions ndi Actin Network mu Inhibiting Cancer Cell Collective Migration. ACS Nano. 2018 Sep 25; 12 (9): 9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Aug 27. PMID: 30118603; PMCID: PMC6156989
