22/03/03Abstract
Ukuqaliswa kwe-AuNRs kunye ne-PPTT kunokuchaphazela uxhulumaniso phakathi kwe-cytoskeleton kunye neseli, ngaloo ndlela inqanda ukufuduka ngokubambisana kweeseli zomhlaza. Ukuphazamiseka kwe-actin cytoskeleton kwaqwalaselwa ngakumbi yi-STORM isebenzisa i-microscope ye-superresolution, ngelixa enye i-microscope yayisetyenziselwa ukurekhoda imifanekiso ye-DIC kwiimvavanyo zokukhulisa (ukuya kwi-200x). Iziphumo zibonise ukuba i-AuNRs ejoliswe kwi-integrin kunye nokusebenzisana kweeseli-cell kunokubangela utshintsho lwe-phosphorylation. Utshintsho kwi-morphology yabo okanye amanqanaba enkcazo, amacandelo aphambili e-cytoskeletal filaments kunye noqhagamshelwano lweeseli, lukwathintela ukufuduka kwethumba.
Umzobo 1 Ukuthelekiswa kwesisombululo se-STORM (A) kunye ne-fluorescence microscopy imaging yesiqhelo (B) ye-actin filaments.
Umzobo 2 Ukuthathwa kweSelula, i-cytotoxicity kunye ne-motility kunyango lwe-AuNRs. (AB) Umahluko
ukuchasana kokuphazamiseka (DIC) imifanekiso emincinci yeeseli ze-HeLa ngaphandle (A) kunye ne
AuNRs@RGD emva kwe 24 h incubation (B). (C) Umfanekiso we-DIC we-AuNRs@RGD usasaze kwiindawo zokuhlangana kweeseli emva kwe-24 h incubation.
Uhlalutyo lweteknoloji yokucinga
I-microscopy ye-fluorescence yendabuko kunzima ukunqumla umda we-optical diffraction, ayikwazi ukujonga umphumo wokungeniswa kwe-nanorods yegolide kunye ne-PPTT kwiiseli, ngaphandle kwesisombululo esiphezulu, akunakwenzeka ukuba umfanekiso wabo.Dhyana 95kwaye400BSIiikhamera ziikhamera ezimbini ezixineneyo, ezinamandla neziguquguqukayo ze-sCMOS ezilungele esi sicelo. Iikhamera zineendlela ezahlukeneyo ezahlukeneyo ezibandakanya imowudi yokufunda ingxolo ephantsi yeCMS kunye nemowudi ephezulu enamandla. I-Backshot sCMOS ayiphumeleli kuphela kufutshane-egqibeleleyo ye-95% ye-quantum esebenzayo, kodwa ikwanebala elikhulu lokujonga njenge-2 ", Impendulo ebanzi ye-spectral ye-200-1100nm inceda ekuphuculeni ubuntununtunu kunye noluhlu oluguquguqukayo lweepikseli ezinkulu ze-11um, ezinye iikhamera ze-sCMOS ezingenazo iingenelo ezininzi. ixesha elide ngaphandle kokubangela ukuguqulwa kwefoto kunye nokufota kweesampuli.
Umthombo weReferensi
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. Igolide yeNanorod yeFotothermal yoNyango iAlters Cell Junctions kunye neNethiwekhi ye-Actin ekuthinteleni ukufuduka kweCell Collective yeCancer. ACS Nano. 2018 Sep 25; 12 (9): 9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Aug 27. PMID: 30118603; I-PMCID: PMC6156989
