22/03/03Abstract
Ukwethulwa kwama-AuNRs kanye ne-PPTT kungathinta ukuxhumana phakathi kwe-cytoskeleton neseli, ngaleyo ndlela kuvimbele ukufuduka okuhlangene kwamaseli omdlavuza. Ukuphazamiseka kwe-actin cytoskeleton kwaphinde kwaqashelwa i-STORM isebenzisa isibonakhulu se-superresolution, kuyilapho esinye isibonakhulu sasetshenziswa ukurekhoda izithombe ze-DIC ekuhlolweni kokukhulisa (kufika ku-200x). Imiphumela ibonise ukuthi ama-AuNR ahloselwe i-integrin kanye nokusebenzisana kwamaseli kungase kubangele izinguquko ze-phosphorylation. Izinguquko kumazinga azo okuma kwazo noma amazwi, okuyizingxenye ezibalulekile ze-cytoskeletal filaments kanye noxhumo lwamaseli, futhi zivimbela ukufuduka kwesimila.
Umdwebo 1 Ukuqhathaniswa kokuxazululwa kwe-STORM (A) kanye nesithombe se-fluorescence microscopy imaging (B) ye-actin filaments.
Umdwebo 2 Ukutholwa kweselula, i-cytotoxicity kanye ne-motility ekwelashweni kwe-AuNRs. (AB) Umehluko
I-interference difference (DIC) izithombe ezincane zamaseli e-HeLa angenayo (A) kanye ne
I-AuNRs@RGD ngemva kokufukamela kwamahora angu-24 (B). (C) Isithombe se-DIC se-AuNRs@RGD sisakaza ezindaweni zokuhlangana kwamaseli ngemva kokufukamela kwamahora angama-24.
Ukuhlaziywa kobuchwepheshe bezithombe
I-microscopy ye-fluorescence yendabuko inzima ukweqa umkhawulo we-optical diffraction, ayikwazi ukubona umphumela wokwethulwa kwama-nanorod egolide kanye ne-PPTT kumaseli, ngaphandle kokuxazulula okukhulu, akunakwenzeka ukuwafanekisa.Dhyana 95futhi400BSIamakhamera angamakhamera amabili e-sCMOS ahlangene, anamandla futhi aguquguqukayo alungele lolu hlelo lokusebenza. Amakhamera anezinhlobo ezahlukene zezindlela ezihlanganisa imodi yokufunda yomsindo ophansi ye-CMS kanye nemodi eguquguqukayo ephezulu. I-Backshot sCMOS ayifinyeleli nje kuphela ngokusebenza kahle kwequantum eseduze-ephelele engu-95%, kodwa futhi inomkhakha omkhulu wokubuka njengo-2", Ukusabela okubanzi kwe-spectral okungu-200-1100nm kulungele ukuthuthukisa ukuzwela nobubanzi obuguquguqukayo bamaphikseli amakhulu angu-11um, amanye amakhamera e-sCMOS angenazo izinzuzo eziningi. isikhathi eside ngaphandle kokudala ukonakala kwezithombe kanye ne-photobleaching yamasampula.
Umthombo wesithenjwa
Wu Y, Ali MRK, Dong B, Han T, Chen K, Chen J, Tang Y, Fang N, Wang F, El-Sayed MA. I-Gold Nanorod Photothermal Therapy Ishintsha Izinhlayiya Zeseli kanye Nenethiwekhi ye-Actin ekuvimbeleni Ukufuduka Kwamangqamuzana Omdlavuza. I-ACS Nano. 2018 Sep 25;12(9):9279-9290. doi: 10.1021/acsnano.8b04128. Epub 2018 Aug 27. PMID: 30118603; I-PMCID: PMC6156989
