Application Challenges
Calcium imaging monitors intracellular free calcium dynamics in real time using fluorescent indicators or genetically encoded calcium indicators (GECIs). Changes in calcium concentration alter fluorescence intensity or emission wavelength, which can be recorded with microscopy systems. Typical applications include monitoring neuronal activity and synaptic transmission in neuroscience, analyzing calcium kinetics in cardiomyocytes, studying cellular signaling pathways, and evaluating pharmacological regulation of calcium channels.
Capturing these rapid calcium transients, which typically occur on the tens to hundreds of milliseconds timescale, requires imaging systems with ultra-high temporal resolution, high sensitivity to detect small ΔF/F changes, excellent signal-to-noise performance, and photostability to minimize phototoxicity during prolonged recordings.
Dhyana 400BSI V3
Classic 6.5 µm BSI sCMOS Camera
Pixel Size: 6.5 µm, optimized for 40×–60× high-NA objectives.
Shutter Modes: Multiple rolling shutter modes, tailored for scanning and light-sheet imaging.
Calibration: PRNU/DSNU correction ensures uniform background for accurate quantitative analysis.
Interface: USB 3.0 and Camera Link.
Cooling: Water + air cooling design for stable, low-noise operation.
Compact Design: Lightweight at 995 g, low power consumption of 45 W.
Aries 6510
Large-Format 6.5 µm BSI sCMOS Camera
Quantum Efficiency: Peak QE up to 95%, readout noise <0.7 e⁻, capable of single-photon measurements.
Pixel Size: 6.5 µm, suitable for Nyquist sampling with 40×–60× high-NA objectives.
Sensor Size: 29.4 mm, 10.2 MP full-resolution frame rate up to 150 fps, enabling high-throughput performance.
Interface: High-speed GigE interface, lossless data transmission, flexible cabling.
Cooling: Highly reliable cooling reduces data fluctuation and improves measurement precision.
Leo 5514 Pro
Large Format High-Throughput BSI sCMOS Camera
Global shutter + BSI sCMOS architecture provides excellent quantitative imaging performance for high-speed weak-light applications
100 G CoF high-speed interface, single interface bandwidth up to 100 Gbps, high-speed and stable, easy to integrate
670 fps @ 14 MP, data throughput increased by 22 times compared to traditional BSI sCMOS cameras
30.5 mm Large FOV, single-frame imaging field of view 2.5 times that of typical 6.5 µm sCMOS
5.5 µm pixel size, better matching the sensitivity and resolution sampling balance for optical systems above 40×
