Application Challenges
Light-sheet microscopy employs thin planar illumination to selectively excite fluorophores within the focal plane, enabling rapid, low-photodamage volumetric imaging. Its advantages include long-term live imaging and three-dimensional visualization of large specimens, making it widely used in studies of embryonic development, neural networks, and whole-organ imaging.
Light-sheet microscopy typically generates massive datasets over extended time periods, placing stringent demands on imaging hardware. Cameras must deliver high speed, Large FOV formats, and long-term stability. Furthermore, to maintain image quality under low excitation light conditions, high sensitivity and low noise are required to support high-throughput, long-duration, and dynamic biological observations.
Dhyana 400BSI V3
Classic 6.5 µm BSI sCMOS Camera
Pixel Size: 6.5 µm, optimized for 40×–60× high-NA objectives.
Shutter Modes: Multiple rolling shutter modes, tailored for scanning and light-sheet imaging.
Calibration: PRNU/DSNU correction ensures uniform background for accurate quantitative analysis.
Interface: USB 3.0 and Camera Link.
Cooling: Water + air cooling design for stable, low-noise operation.
Compact Design: Lightweight at 995 g, low power consumption of 45 W.
Aries 6510
Large-Format 6.5 µm BSI sCMOS Camera
Quantum Efficiency: Peak QE up to 95%, readout noise <0.7 e⁻, capable of single-photon measurements.
Pixel Size: 6.5 µm, suitable for Nyquist sampling with 40×–60× high-NA objectives.
Sensor Size: 29.4 mm, 10.2 MP full-resolution frame rate up to 150 fps, enabling high-throughput performance.
Interface: High-speed GigE interface, lossless data transmission, flexible cabling.
Cooling: Highly reliable cooling reduces data fluctuation and improves measurement precision.
Leo 5514 Pro
Leo 5514 Pro
Large Format High-Throughput BSI sCMOS Camera
Global shutter + BSI sCMOS architecture provides excellent quantitative imaging performance for high-speed weak-light applications
100 G CoF high-speed interface, single interface bandwidth up to 100 Gbps, high-speed and stable, easy to integrate
670 fps @ 14 MP, data throughput increased by 22 times compared to traditional BSI sCMOS cameras
30.5 mm Large FOV, single-frame imaging field of view 2.5 times that of typical 6.5 µm sCMOS
5.5 µm pixel size, better matching the sensitivity and resolution sampling balance for optical systems above 40×
